FastQCFastQC Report
Mon 6 Feb 2017
SRR2079203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4077196
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71000.040.0994641
GGTATCA29250.037.3730351
ATCAACG104550.028.1254223
TCAACGC105700.027.6235084
TATCAAC103950.027.4577222
CAACGCA108600.026.5998465
AACGCAG113250.025.4520246
ACGCAGA135100.021.1500077
CGCAGAG138900.020.4190658
GAGTACT89950.019.48579612
AGAGTAC133450.019.05480611
GTACATG100000.018.853571
CAGAGTA139950.018.51495610
AGTACTT95950.018.19136413
TACATGG101650.018.1647682
GTACTTT98450.017.8056114
GCAGAGT151500.017.718329
ACATGGG105400.017.1910423
TATACTG14050.016.4581855
ACTTTTT117500.015.94670116
Produced by FastQC (version 0.11.5)