FastQCFastQC Report
Mon 6 Feb 2017
SRR2079202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648606
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38600.032.624241
GGTATCA21350.028.0333231
TCAACGC46850.026.2128684
ATCAACG48000.025.8723223
CAACGCA47750.025.1411975
TATCAAC51550.024.045132
AACGCAG51350.023.1099016
ACGCAGA58950.018.959097
CGCAGAG61050.018.0249148
GTACATG72950.017.6892681
GTATAGC12450.017.5064281
TACATGG74050.016.9255542
AGAGTAC59900.016.07445111
ACATGGG75800.015.973943
TATACTG13450.015.3889525
GTGTAGC20950.015.1925321
CAGAGTA66400.014.65682110
GAGTACT30650.013.96169212
GTGGTAT14400.013.9345151
TAGCCCT23400.013.857544
Produced by FastQC (version 0.11.5)