FastQCFastQC Report
Mon 6 Feb 2017
SRR2079201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022659
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36500.032.3132061
GGTATCA19700.032.129461
TCAACGC47050.025.8107414
ATCAACG46800.025.5063113
CAACGCA48950.024.9498545
TATCAAC48450.024.4467142
AACGCAG50500.023.9791166
ACGCAGA58400.020.380247
CGCAGAG59750.019.7472068
GTACATG70500.019.3787861
TACATGG70100.018.9654372
AGAGTAC57350.017.57011811
ACATGGG74950.017.169383
CAGAGTA61950.016.65540310
GAGTACT27600.016.50348912
ACCGTGC5550.016.16218
GCAGAGT66750.016.1300539
CATGGGG58700.015.2222584
GAGTACA53400.015.0915291
CCTATAC6000.014.9499423
Produced by FastQC (version 0.11.5)