FastQCFastQC Report
Mon 6 Feb 2017
SRR2079201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022659
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53950.040.2554631
GGTATCA22000.036.3116461
TATCAAC77800.027.8146762
ATCAACG79950.027.4120373
TCAACGC80650.027.131324
CAACGCA82450.026.5390075
AACGCAG86500.025.340526
ACGCAGA103050.020.9292357
CGCAGAG104850.020.4362588
GAGTACT68650.019.75221812
AGAGTAC100900.019.49160211
AGTACTT72050.018.76850713
CAGAGTA109300.018.46713610
GTACATG69400.018.3377041
GTACTTT74850.018.20738614
GCAGAGT112750.018.0553749
TACATGG69350.017.7667432
ACATGGG73300.016.6680723
TACTTTT87200.015.62870215
ACTTTTT93200.015.28890816
Produced by FastQC (version 0.11.5)