FastQCFastQC Report
Mon 6 Feb 2017
SRR2079200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163223
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34200.034.0912321
GGTATCA18100.029.6272951
TCAACGC43900.026.5627824
ATCAACG44800.026.0291563
CAACGCA45250.025.694065
TATCAAC45300.025.4613842
AACGCAG49500.023.6970946
GCGCTAG2650.022.1192560
AGCGCTA3050.021.47968559
GTACATG74950.020.2181471
TACATGG75400.019.2816622
ACGCAGA59000.019.2370197
CGCAGAG61650.018.019568
ACATGGG79350.017.5653113
AGAGTAC57600.016.59727311
GTATTGG10650.016.5675951
CGCTAGA3601.8189894E-1216.2827461
CATGGGG59350.015.3463324
CAGAGTA63900.015.28498410
TATACAG13300.014.26699355
Produced by FastQC (version 0.11.5)