FastQCFastQC Report
Mon 6 Feb 2017
SRR2079200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163223
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47400.037.4391861
GGTATCA21950.035.6173251
TCAACGC66300.026.6559894
TATCAAC65850.026.6284732
ATCAACG67000.026.4290143
CAACGCA68400.025.938535
AACGCAG73200.024.146776
ACGCAGA89050.019.4159987
CGCAGAG91350.018.9613258
GTACATG74750.018.7426591
GAGTACT52600.018.30143412
AGAGTAC87450.018.18895711
TACATGG75300.017.6483522
TATACGG1402.0760065E-417.2587135
CAGAGTA94950.016.97026310
AGTACTT57050.016.5688713
GTACTTT58600.016.36894414
ACATGGG81100.016.3436373
CCAATAC8300.016.219033
GCAGAGT104250.016.019029
Produced by FastQC (version 0.11.5)