FastQCFastQC Report
Mon 6 Feb 2017
SRR2079199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518529
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47200.034.2370871
GGTATCA24550.030.8684311
TCAACGC63050.025.2215064
ATCAACG63550.024.9687883
TATCAAC62850.024.7233472
CAACGCA64550.024.5823275
AACGCAG67950.023.301546
ACGCAGA77750.019.6099787
GTACATG98450.018.9450151
CGCAGAG81300.018.8390928
TACATGG99250.018.4393392
CGAATCG2458.058123E-1018.30290837
AGAGTAC75800.017.43668411
ACATGGG108800.016.4231343
CAGAGTA84350.016.24200810
GTATAGG7650.015.8317361
GCAGAGT89350.015.7965799
TATACTG11400.015.4321585
GCGATAC3151.3569661E-915.33652914
GAGTACT28350.014.97179912
Produced by FastQC (version 0.11.5)