FastQCFastQC Report
Mon 6 Feb 2017
SRR2079199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518529
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68650.041.706431
GGTATCA26450.036.7361681
TATCAAC96300.029.3856642
ATCAACG98050.028.8963833
TCAACGC98500.028.6942984
CAACGCA101200.027.621835
AACGCAG105700.026.4841736
ACGCAGA124650.022.204577
CGCAGAG128600.021.3578878
GAGTACT78050.020.468612
AGAGTAC123100.020.32169711
GTACATG97400.019.9166891
AGTACTT81200.019.67008213
TACATGG99850.019.4930132
CAGAGTA133150.019.17655610
GTACTTT83000.018.99952914
GCAGAGT139800.018.6099059
ACATGGG106050.018.0279793
TACTTTT93400.016.92113515
ACTTTTT99400.016.5940516
Produced by FastQC (version 0.11.5)