FastQCFastQC Report
Mon 6 Feb 2017
SRR2079198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543183
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.035.7401661
GTATCAA55600.034.8471
TCAACGC70200.027.129274
ATCAACG72050.026.480573
CAACGCA72650.026.1676315
TATCAAC72850.026.118292
AACGCAG75700.024.7942756
CGCAGAG87450.020.9496618
ACGCAGA88050.020.8457957
GTACATG95200.020.6425611
AGAGTAC84150.019.15476611
TACATGG96000.019.0645852
CAGAGTA91600.017.89831710
GCAGAGT95450.017.8639439
GAGTACT44150.017.74460212
ACATGGG101600.017.4883843
GAGTACA69650.015.6970521
GTGTAGC18200.015.5881521
AGTACTT51600.015.24909213
GTATAGA9550.015.2159171
Produced by FastQC (version 0.11.5)