FastQCFastQC Report
Mon 6 Feb 2017
SRR2079198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543183
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73300.041.759061
GGTATCA27200.038.5287671
ATCAACG107150.028.8304963
TCAACGC108600.028.572694
TATCAAC107300.028.4685132
CAACGCA111200.027.5942275
AACGCAG115250.026.6002246
ACGCAGA139700.021.5923297
CGCAGAG141000.021.363048
GAGTACT94750.020.31939112
AGAGTAC140550.019.93337611
TTTCGCG900.00574235519.16341422
CAGAGTA146950.019.15915110
AGTACTT100100.019.12566413
GTACATG95350.019.1161881
GCAGAGT150600.018.8093559
TACATGG94900.018.6946852
GTACTTT104600.018.20932214
ACATGGG102600.016.9216183
TACTTTT119200.016.32681515
Produced by FastQC (version 0.11.5)