FastQCFastQC Report
Mon 6 Feb 2017
SRR2079197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654282
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44400.034.830551
GGTATCA21200.032.8016741
TCAACGC55850.026.7462464
ATCAACG56900.026.3739433
CAACGCA57700.026.0680645
TATCAAC59650.025.4128532
AACGCAG61100.024.5045456
ACGCAGA71200.020.640087
CGCAGAG70800.020.5621788
GTACATG82150.020.4674851
TACATGG83050.019.458332
GAGTACT28950.018.8341412
AGAGTAC68300.018.5441611
ACATGGG86900.018.2614883
CAGAGTA72750.017.97910310
GCAGAGT75700.017.6431839
TACACCG2451.5901605E-816.8971425
CATGGGA43150.016.8694044
TATACTG10800.016.6103085
AGTACTT35800.015.61568613
Produced by FastQC (version 0.11.5)