FastQCFastQC Report
Mon 6 Feb 2017
SRR2079197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654282
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.1363457236269545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77750.041.545431
GGTATCA30450.039.183981
TATCAAC112050.028.6453862
ATCAACG113550.028.327773
TCAACGC114450.027.8939194
CAACGCA117800.026.9541825
AACGCAG123050.025.7529246
TATGCCG3400.023.33476343
ACGCAGA145600.021.4734237
CGCAGAG147000.021.0540668
GAGTACT99950.020.81512512
AGAGTAC141900.020.2781411
TCTCGTA4700.019.816238
CGTGCGC3850.019.71543910
CAGAGTA148750.019.71543710
AGTACTT107050.019.4631413
GTACTTT108650.019.1837514
GCAGAGT153300.019.175299
GTACATG85850.018.9739321
TACATGG85550.018.5979252
Produced by FastQC (version 0.11.5)