FastQCFastQC Report
Mon 6 Feb 2017
SRR2079196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745984
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57800.036.9861341
GGTATCA26450.035.1807331
ATCAACG77150.027.811893
TCAACGC76300.027.7600274
TATCAAC78150.027.2133332
CAACGCA77900.027.057375
AACGCAG81650.025.856946
ACGCAGA92950.022.1561977
CGCAGAG96400.021.2919778
GTACATG95700.020.6396561
AGAGTAC89600.020.41379511
GAGTACT48050.019.3908712
TACATGG98900.019.2346952
CAGAGTA96550.019.08731710
GCAGAGT100600.018.6609449
CGACACG2053.8562575E-818.50097368
ACATGGG99900.018.301493
AGTACTT54500.017.60206413
GTGTAGC17200.016.692751
GTGGTAT14450.016.5180781
Produced by FastQC (version 0.11.5)