FastQCFastQC Report
Mon 6 Feb 2017
SRR2079196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745984
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.041.7842031
GTATCAA84050.040.5337641
ATCAACG120950.028.6791953
TCAACGC121950.028.2459074
TATCAAC120250.028.1859842
CAACGCA126050.027.080725
AACGCAG130100.026.1099346
ACGCAGA154100.021.7226127
CGCAGAG157550.021.134318
GAGTACT109100.020.05108512
AGAGTAC152700.019.92979811
TATACCG2505.0931703E-1119.328355
AGTACTT115500.019.11544413
CAGAGTA163650.018.828210
GTACATG95200.018.7469731
GCAGAGT167900.018.5982159
GTACTTT117350.018.5838814
TACATGG95500.017.8899272
ACATGGG100650.016.9745463
TACTTTT134250.016.27015915
Produced by FastQC (version 0.11.5)