FastQCFastQC Report
Mon 6 Feb 2017
SRR2079195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941104
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.035.5603331
GTATCAA37550.035.479891
TCAACGC49400.026.9526254
ATCAACG50000.026.8361593
CAACGCA50150.026.343655
TATCAAC51250.025.5337542
AACGCAG52400.025.344146
ACGCAGA62000.020.975517
GTACATG82700.020.503221
CGCAGAG64300.020.2259088
TACATGG81600.020.183552
ACATGGG87200.018.6313863
AGAGTAC61300.018.52368211
CGTGCCG2105.021866E-818.07862310
TATACCG1753.2162498E-617.7399585
CAGAGTA66100.017.64847410
GCAGAGT71750.016.7397619
GAGTACT25250.016.67480712
TATTCCG2502.0434527E-816.5572935
GTATAGG6400.016.2208181
Produced by FastQC (version 0.11.5)