FastQCFastQC Report
Mon 6 Feb 2017
SRR2079195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941104
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.041.535541
GTATCAA61600.040.878581
TATCAAC90150.027.8698482
ATCAACG93400.027.2695793
TCAACGC92700.027.2521214
CAACGCA94300.026.4969465
AACGCAG97850.025.5761266
GTACATG79300.021.2422681
ACGCAGA116750.021.1653337
CGCAGAG119650.020.5612628
TACATGG81350.020.4059332
GAGTACT78700.020.03581412
AGAGTAC114150.019.7077611
ACATGGG85350.019.6113343
AGTACTT83500.018.96250713
CAGAGTA121050.018.92643410
GTACTTT86000.018.095314
GCAGAGT128650.018.0497369
CGAATCG2502.0427251E-816.55782937
TATGCCG4200.016.42613843
Produced by FastQC (version 0.11.5)