FastQCFastQC Report
Mon 6 Feb 2017
SRR2079194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008654
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42200.035.751721
GGTATCA20800.030.6109921
TCAACGC54400.027.2064654
TATCAAC55350.027.0804312
ATCAACG55900.026.5998523
CAACGCA55150.026.5862545
AACGCAG59200.025.2336436
ACGCAGA67800.021.167537
CGCAGAG69450.020.366928
GTACATG76200.020.1628041
AGAGTAC65300.019.3443911
TACATGG78950.019.1166922
ACATGGG81800.018.557233
CAGAGTA71200.018.03225710
GAGTACT29550.017.63538212
GCAGAGT76700.017.4141679
GTATAGG8500.016.284111
TACGCTC3502.1827873E-1115.76808834
TACGCTA1350.003236321315.3392679
GTGGTAT13250.015.1472941
Produced by FastQC (version 0.11.5)