FastQCFastQC Report
Mon 6 Feb 2017
SRR2079194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008654
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.040.3718871
GTATCAA60950.039.675221
TCAACGC86850.027.6649444
ATCAACG87500.027.6566963
TATCAAC86800.027.6013362
CAACGCA89300.026.828625
AACGCAG93100.025.8111696
ACGCAGA111550.020.97457
CGCAGAG114800.020.3175478
GAGTACT71300.020.17895112
GTACATG77450.019.8324991
AGAGTAC109400.019.45897111
TCGATCG2153.1595846E-919.25279441
AGTACTT75750.019.17284413
CAGAGTA114650.018.8387610
TACATGG81250.018.694752
GTACTTT78550.018.27255214
GCAGAGT122300.017.9707019
ACATGGG84700.017.3219153
ACTTTTT93900.016.42456816
Produced by FastQC (version 0.11.5)