FastQCFastQC Report
Mon 6 Feb 2017
SRR2079193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303488
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.037.9914131
GTATCAA35750.035.1061061
TCAACGC47000.026.9392534
ATCAACG46900.026.628893
CAACGCA48200.026.4122925
AACGCAG50550.025.1161696
TATCAAC50400.024.8653952
GTACATG77800.021.6422041
ACGCAGA59450.020.7168757
TACATGG78000.020.3159582
CGCAGAG61150.019.971258
TATACCG1258.7446926E-519.3203415
ACATGGG81200.019.2043553
AGAGTAC58250.018.12746811
CAGAGTA61900.017.56028610
GTATAGC7300.017.5239091
CGTTAGG1200.001470563617.2619272
GCAGAGT66450.016.7213959
GTATAGG5850.016.5483341
CATGGGG42750.016.5437934
Produced by FastQC (version 0.11.5)