FastQCFastQC Report
Mon 6 Feb 2017
SRR2079193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303488
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.10744575183374083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.043.4355051
GTATCAA64450.041.6920551
TATCAAC93550.028.4782312
ATCAACG95250.028.3684923
TCAACGC96150.027.9952814
CAACGCA98800.027.349185
AACGCAG103150.026.2332636
ACGCAGA121700.021.890087
CGCAGAG123900.021.2205758
GAGTACT84650.020.25741612
CGTATAC1206.3770305E-520.1305793
AGAGTAC121050.019.78104811
AGTACTT88500.019.60667213
CAGAGTA124650.019.37583410
GCAGAGT128900.018.977899
GTACATG76950.018.6956231
GTACTTT92000.018.56442314
TACATGG78450.018.1675592
GTATAGG6100.018.1415921
TAATTCG1954.6034984E-717.69029859
Produced by FastQC (version 0.11.5)