FastQCFastQC Report
Mon 6 Feb 2017
SRR2079192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528644
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32550.032.7438661
GGTATCA17450.028.1593861
ATCAACG38350.025.8220273
TCAACGC38950.025.3356674
CAACGCA39200.024.9980455
TATCAAC40550.024.8696612
AACGCAG41100.024.0942756
ACGCAGA45800.020.9436847
CGCAGAG47050.020.1676588
GTACATG53200.018.4079361
AGAGTAC43400.018.29150411
TACATGG52100.018.0305522
GTACTAG5650.017.7615071
CAGAGTA49650.016.8926710
GAGTACT21700.016.85799212
GCAGAGT53200.016.4790979
GTGTAGC12900.016.3632811
ACATGGG55600.015.9489513
ACGTTAG1350.003242615115.33465748
CGGGCGT2302.583165E-614.99625129
Produced by FastQC (version 0.11.5)