FastQCFastQC Report
Mon 6 Feb 2017
SRR2079192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528644
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45250.039.1294941
GGTATCA18750.033.3833661
TATCAAC61150.029.0626222
TCAACGC61550.028.6494884
ATCAACG62300.028.5261543
CAACGCA63300.027.8029255
AACGCAG66350.026.4250346
ACGCAGA77500.021.9949767
CGCAGAG80950.020.7993478
AGAGTAC77150.019.90081211
GAGTACT53700.019.78896912
AGTACTT56600.018.71112813
CAGAGTA84700.018.371310
TATATCG950.00784036318.15653866
GCAGAGT86650.018.1171369
GTACTTT58550.017.85616714
GTACATG54450.016.57661
ACGTCCG1250.001942964516.55810737
TACATGG54700.015.8347222
ACTTTTT70750.015.65492916
Produced by FastQC (version 0.11.5)