FastQCFastQC Report
Mon 6 Feb 2017
SRR2079191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002124
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37650.035.6498761
GGTATCA18000.033.82451
ATCAACG48000.026.5936343
TCAACGC47500.026.437784
TATCAAC50450.025.6000232
CAACGCA49250.025.428745
AACGCAG50950.024.6484076
ACGCAGA60300.020.2543187
GTACATG72400.018.9689451
CGCAGAG63400.018.9374588
AGAGTAC60150.018.24388311
TACATGG72750.018.085142
CAGAGTA66100.017.17592810
ACATGGG77150.016.9927983
GTATAGC8700.016.7001841
GCAGAGT70950.015.9537119
GTGTAGC13650.015.2058191
GTATAGG7400.014.9592381
GTAACGC1400.004116565514.79452527
TAGTCGG1400.004138975414.78244840
Produced by FastQC (version 0.11.5)