FastQCFastQC Report
Mon 6 Feb 2017
SRR2079191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002124
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57900.039.483041
GGTATCA25050.038.3817521
TCAACGC83850.027.5783564
ATCAACG84100.027.4963763
TATCAAC82650.027.3941362
CAACGCA85800.026.6297655
AACGCAG89450.025.8158156
ACGCAGA105850.021.3867367
CGCAGAG107950.020.8063458
GAGTACT73500.019.9964312
GTACATG71950.019.707921
TACATGG72150.019.2781492
AGAGTAC104350.019.27552811
AGTACTT77800.018.71166613
CAGAGTA111950.018.39841710
GTACTTT80400.018.28214614
GCAGAGT115200.018.1791529
ACATGGG77150.017.9392833
CATGGGG53800.016.2947644
TCTAGCG1503.5312842E-416.0977357
Produced by FastQC (version 0.11.5)