FastQCFastQC Report
Mon 6 Feb 2017
SRR2079190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425562
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46500.033.4717451
GGTATCA25300.033.2203751
TCAACGC60800.025.5331084
TATCAAC61750.024.9942232
ATCAACG62350.024.8430353
CAACGCA61900.024.7449785
AACGCAG65350.023.7025746
ACGCAGA76050.019.6407497
GTACATG94850.019.4725441
CGCAGAG78850.018.5500978
TACATGG95350.018.5041582
AGAGTAC74450.017.66015811
ACATGGG101350.017.4277363
GTGGTAT13850.016.7318461
CAGAGTA81850.016.31648410
GAGTACT30450.015.97867412
GCAGAGT87750.015.7304379
GTCCTAC7150.014.9959661
TACACCG3705.4569682E-1114.9180825
CATGGGG70700.014.8824764
Produced by FastQC (version 0.11.5)