FastQCFastQC Report
Mon 6 Feb 2017
SRR2079190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425562
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72100.040.8812751
GGTATCA29400.039.302421
TATCAAC104000.028.3091052
ATCAACG107150.027.9600543
TCAACGC108050.027.6313294
CAACGCA109400.027.195715
AACGCAG114650.025.8348626
ACGCAGA133350.021.9208017
CGCAGAG135900.021.4044678
AGAGTAC130500.020.4383711
GTACATG92250.020.1760441
GAGTACT87800.020.00324812
CAGAGTA137350.019.59490610
TACATGG94500.019.1385882
GCAGAGT143950.019.128519
AGTACTT93300.018.81994213
GTACTTT95100.018.4314914
ACATGGG100850.017.4886153
GTACCGA5650.017.108136
TCCGTTA3551.8189894E-1216.51948265
Produced by FastQC (version 0.11.5)