FastQCFastQC Report
Mon 6 Feb 2017
SRR2079188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089899
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59300.027.011591
GGTATCA35150.024.409011
TCAACGC66850.022.5004234
ATCAACG67550.022.4715443
CAACGCA69150.021.5524775
GTACATG86950.021.4458371
TATCAAC73250.020.6474022
TACATGG90700.020.0632932
AACGCAG73600.020.0150056
ACATGGG93700.018.556523
ACGCAGA81700.017.5230797
CGCAGAG82800.017.2902858
AGAGTAC73650.016.96375811
GTATACG2057.54384E-716.8760171
GTATAGG11050.015.9673091
TATACCG2603.308196E-815.9225845
GAGTACT39150.015.86720612
CATGGGG60050.015.5115924
TGCCGTA2705.2383257E-815.33811115
TAGTTCG1808.072803E-515.33792448
Produced by FastQC (version 0.11.5)