FastQCFastQC Report
Mon 6 Feb 2017
SRR2079188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089899
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91800.036.0883031
GGTATCA40750.028.8532621
ATCAACG120450.027.220353
TCAACGC121100.026.9887474
TATCAAC122550.026.6412562
CAACGCA124150.026.1589185
AACGCAG129200.025.0068686
ACGCAGA152800.020.8675947
CGCAGAG152950.020.7084668
AGAGTAC142450.020.07848211
GTACATG95750.019.8640381
GAGTACT103500.019.50088312
TACATGG97100.019.228922
AGTACTT108950.018.6488713
CAGAGTA159850.018.56199510
GCAGAGT167000.018.0565099
GTACTTT112000.018.02200914
ACATGGG98850.017.8759943
TCGATCG1804.2065276E-617.24783141
CGTTACG2301.4273792E-716.49792524
Produced by FastQC (version 0.11.5)