FastQCFastQC Report
Mon 6 Feb 2017
SRR2079187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3031335
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.033.6739731
GTATCAA44650.032.230581
ATCAACG59850.024.323413
TCAACGC59300.024.3163154
TATCAAC60700.023.3788782
CAACGCA61200.023.2795645
AACGCAG63900.022.4578746
CGCGTAA900.005743095719.16287836
ACGCAGA72950.019.0570627
GTACATG83150.018.6801511
CGCAGAG73600.018.5141018
GTATAGA7650.017.6359751
TACATGG83950.017.4387652
ACATGGG86250.016.8783323
AGAGTAC68850.016.59071511
GAGTACT31500.016.10425812
GCAGAGT78900.015.5711699
CAGAGTA76650.015.17255810
TAGACCG2152.2673867E-514.44043355
CATGGGG58650.014.2927054
Produced by FastQC (version 0.11.5)