FastQCFastQC Report
Mon 6 Feb 2017
SRR2079187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3031335
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73450.040.078611
GGTATCA29900.039.682391
TATCAAC101000.029.0447642
TCAACGC102700.028.900034
ATCAACG104050.028.7572483
CAACGCA105550.027.98895
AACGCAG110600.026.6230246
ACGCAGA129350.022.3595077
CGCAGAG130950.021.9509078
GAGTACT88750.021.73268712
AGAGTAC127300.020.8433711
AGTACTT95350.020.58515213
GCAGAGT136300.019.973699
CAGAGTA134800.019.91405510
GTACTTT99350.019.58759914
CTCGTAT4500.019.16385339
GTACATG84450.018.80131
TACATGG85550.018.5166742
TACTTTT106600.018.19068715
TATGCCG4800.017.24746943
Produced by FastQC (version 0.11.5)