FastQCFastQC Report
Mon 6 Feb 2017
SRR2079186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722415
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32650.032.758721
GGTATCA16200.031.1953181
ATCAACG39600.026.2240493
TCAACGC39150.026.172984
CAACGCA40600.025.493165
TATCAAC42000.024.8304962
AACGCAG42800.024.0215476
ACGCAGA49650.020.0808967
GTACATG57650.019.9938531
CGCAGAG50900.019.5881128
TACATGG57200.019.4999832
ACATGGG60900.018.3550763
ACCGCGT950.00783782118.1576188
AGAGTAC47350.017.92831211
CATACCG2156.600021E-817.6515435
GTATAGC8250.017.20211
CAGAGTA50650.017.16901410
GAGTACT25500.016.10271512
GAGTACG2201.5676778E-615.7336291
CGCAACG1554.52115E-415.58327217
Produced by FastQC (version 0.11.5)