FastQCFastQC Report
Mon 6 Feb 2017
SRR2079186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722415
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.042.2169841
GTATCAA49100.038.5862961
TATCAAC67150.027.9080222
ATCAACG69000.027.6599393
TCAACGC69550.027.4412064
CAACGCA71100.026.3575735
AACGCAG72700.025.7841226
ACGCAGA87450.020.9553037
CGCAGAG88600.020.5626778
ACGCGGA1409.325051E-619.7206487
AGAGTAC86450.019.637211
GAGTACT62100.019.50271212
CAGAGTA90150.019.02261210
TACATGG60100.019.0076642
GTACATG60550.018.8993931
AACGCGG1651.8194605E-618.8297926
GCAGAGT93950.018.473569
GTACTTT67300.018.45792414
AGTACTT66700.018.25646213
ACATGGG62700.017.7791183
Produced by FastQC (version 0.11.5)