FastQCFastQC Report
Mon 6 Feb 2017
SRR2079185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032520
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.032.1237031
TCGTACG450.004631730330.65602331
TCAACGC39000.026.802944
GGTATCA18600.026.779911
CAACGCA39150.026.3477695
ATCAACG39650.026.276543
AACGCAG42500.024.5144676
TATCAAC42350.024.3782272
ACGCAGA50500.020.0161577
GTACATG64750.019.4990141
CGCAGAG51750.018.8660328
TACATGG64150.018.3006532
GCGATAC1552.2448336E-517.80937814
ACATGGG68250.016.8829483
AGAGTAC51800.016.25658611
GAGTACT26200.015.41054412
CAGAGTA58350.015.08233510
GCAGAGT60600.015.0349039
GTACGGG2152.209823E-514.4798351
CATGGGG48850.013.9831514
Produced by FastQC (version 0.11.5)