FastQCFastQC Report
Mon 6 Feb 2017
SRR2079185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032520
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52950.038.2188221
GGTATCA23500.034.8873441
TCAACGC71650.027.8402674
ATCAACG72150.027.6951663
TATCAAC72900.027.4102342
CAACGCA73300.027.0723275
AACGCAG76650.025.9416
ACGCAGA88250.022.2123937
CGCAGAG89900.021.6472328
AGAGTAC88350.019.95709411
GAGTACT60450.019.8068812
GTACATG63550.018.9431021
GCAGAGT97050.018.9146829
CAGAGTA95250.018.90986610
TACATGG63950.018.4564112
TCGATCG1702.4384335E-618.2619741
AGTACTT66000.018.03198413
GTACTTT66500.017.69474614
ACATGGG68000.016.7988993
TATAGCG1452.717445E-416.6606455
Produced by FastQC (version 0.11.5)