FastQCFastQC Report
Mon 6 Feb 2017
SRR2079184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597350
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.039.22661
GTATCAA48150.034.9303671
TATCAAC61300.026.649682
TCAACGC61250.026.5330754
ATCAACG62650.026.2155323
CAACGCA63300.025.4012475
AACGCAG65250.024.695016
GTACATG98250.022.4372711
TACATGG96050.021.8264272
ACGCAGA76300.020.7564267
ACATGGG101850.020.4959073
CGCAGAG79200.019.691458
CGCAATT2002.8932845E-818.9700434
AGAGTAC75500.018.37570211
CATGGGG59050.017.9971474
GAGTACT32950.017.90902912
CAGAGTA80050.017.46057310
CATGGGA43050.017.0717964
GTGGTAT11550.016.7791211
GCAGAGT85450.016.6809049
Produced by FastQC (version 0.11.5)