FastQCFastQC Report
Mon 6 Feb 2017
SRR2079184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597350
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.043.444781
GTATCAA73850.040.92891
TATCAAC101300.029.6431882
ATCAACG106450.028.7926983
TCAACGC106450.028.7602754
CAACGCA110750.027.5501295
AACGCAG114650.026.7671036
ACGCAGA134100.022.518317
CGCAGAG136250.021.9299478
TACATGG100700.021.1137962
GTACATG103400.020.8036251
GAGTACT82300.020.49974612
AGAGTAC134000.020.28901111
TAAGACG1206.368897E-520.1340854
CAGAGTA138850.019.77910610
AGTACTT86400.019.64372813
GCAGAGT143100.019.5292199
ACATGGG110100.019.2171173
GTACTTT91250.018.52939214
CGCACGT1200.00147889817.24748852
Produced by FastQC (version 0.11.5)