FastQCFastQC Report
Mon 6 Feb 2017
SRR2079183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3572071
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44500.034.5170941
GGTATCA22650.032.99111
TCAACGC57450.026.782434
ATCAACG58500.026.4786383
CAACGCA58750.025.7203875
TATCAAC59950.025.2274592
AACGCAG62550.024.5990776
ACGCAGA73250.020.5810177
CGCAGAG75250.019.3921898
AGAGTAC72450.017.72215811
GTACATG79550.017.4822431
TACATGG80700.016.4303282
CAGAGTA81050.016.13980310
GCAGAGT84000.016.0656159
ACATGGG82000.015.9031483
GAGTACT39300.015.54354712
CGACGTT2101.8072347E-514.79166246
GTACTTT43900.014.54335114
GTATAGC10900.014.2822721
AGTACTT44450.014.20851813
Produced by FastQC (version 0.11.5)