FastQCFastQC Report
Mon 6 Feb 2017
SRR2079183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775876
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30700.040.6617551
GGTATCA12500.039.8354531
ATCAACG44750.027.9276643
TATCAAC44600.027.7119622
TCAACGC44250.027.6970924
CAACGCA46900.026.0585085
AACGCAG49400.024.9494136
TATACCG1004.3159202E-420.7143045
ACGCAGA59500.020.3581337
GCGCTAT1206.2775995E-520.1713091
GTACATG39050.020.0126531
ATACCGG1055.9967925E-419.727916
CGCAGAG61450.019.6515188
GAGTACT39850.018.95852712
AGAGTAC58500.018.39871611
TACATGG41550.018.03052
AGTACTT43450.017.46713413
CAGAGTA63400.017.35762210
GCAGAGT65150.017.3149859
GTATAGC5000.017.2896941
Produced by FastQC (version 0.11.5)