FastQCFastQC Report
Mon 6 Feb 2017
SRR2079182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885761
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.035.2987441
GGTATCA22850.033.3059461
TCAACGC60300.028.377464
ATCAACG61100.027.9494423
CAACGCA62800.027.2477845
TATCAAC61600.027.2454592
AACGCAG63750.026.679396
ACGCAGA74250.022.4879867
CGCAGAG76150.021.6097688
GTACATG94200.020.3443681
AGAGTAC74000.020.19405211
TACATGG92400.020.181822
ACATGGG96050.019.467543
CAGAGTA77800.019.42950810
GCAGAGT82300.018.5768139
CATGGGG61800.018.0869664
GCGACTA1150.001108235118.00614411
GAGTACT30950.017.95156912
TATACCG1653.845586E-516.726895
AGTACTT35250.015.95725313
Produced by FastQC (version 0.11.5)