FastQCFastQC Report
Mon 6 Feb 2017
SRR2079182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885761
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.13400278124210563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.043.733421
GTATCAA80500.041.842891
TATCAAC113200.029.905722
ATCAACG117850.029.0771163
TCAACGC118350.028.8084814
CAACGCA121050.028.0233735
AACGCAG123550.027.3796526
ACGCAGA149000.022.4906647
CGCAGAG150100.022.1372978
CGCGGTA800.002903121321.5591253
GAGTACT105650.021.00005512
AGAGTAC147650.020.98562211
CAGAGTA153500.020.41063310
AGTACTT111350.020.13674413
GCAGAGT158700.019.9810169
GTACATG92650.019.3349441
TACATGG90800.019.2307552
GTACTTT116150.019.10230614
ACATGGG94050.018.0158353
TACTTTT127550.017.44941315
Produced by FastQC (version 0.11.5)