FastQCFastQC Report
Mon 6 Feb 2017
SRR2079181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137509
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39350.030.338491
GGTATCA21600.028.836221
TCAACGC48000.024.6533184
ATCAACG48050.024.4840643
CAACGCA49800.023.7626155
TATCAAC51000.022.9548632
AACGCAG52400.022.3201876
ACGCAGA58850.019.2286997
CGCAGAG60750.018.4569368
GTACATG61400.017.6398751
AGAGTAC57400.017.6183511
GTATAGC11100.016.8341161
TACATGG61050.016.8002592
ACATGGG62900.016.1257233
CGTCGGA1300.00251805415.92777417
CAGAGTA64550.015.82723310
GAGTACT33750.015.44109412
GCAGAGT67100.015.3288749
TATACCG2302.5763056E-615.0003575
GTATAGG7900.014.8926291
Produced by FastQC (version 0.11.5)