FastQCFastQC Report
Mon 6 Feb 2017
SRR2079181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137509
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28150.041.650071
GTATCAA65350.037.2581671
TATCAAC91450.026.4935262
ATCAACG93500.026.4294283
TCAACGC94050.026.1280844
CAACGCA96300.025.4100955
AACGCAG98850.024.7922846
ACGCAGA116950.020.5364867
ATCCGCG850.004122066320.29384411
CGCAGAG118850.020.000798
GAGTACT90250.019.03686312
AGAGTAC116950.018.73211311
CAGAGTA123350.018.12379510
AGTACTT97450.017.80536813
GCAGAGT127750.017.688899
GTACTTT99500.017.40857114
GTACATG63050.016.8950731
TACATGG62650.016.5818042
GTATAGA10100.016.0942571
TAGACGG1554.513987E-415.5866475
Produced by FastQC (version 0.11.5)