FastQCFastQC Report
Mon 6 Feb 2017
SRR2079180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086259
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.032.2627261
GTATCAA43200.031.4750161
TCAACGC54400.024.9243624
ATCAACG54550.024.792583
TATCAAC54550.024.3743532
CAACGCA57600.023.5396775
AACGCAG58350.022.9414736
GTACATG73650.019.260531
ACGCAGA67150.019.0612477
TCGATCG1852.6508678E-718.6458141
CGCAGAG69200.018.4467148
TACATGG73150.018.4126972
CGATCGT2105.05388E-818.06867842
AGAGTAC64250.017.3506111
ACATGGG77350.016.7263573
CGTCGTT2301.4217039E-716.50337446
CAGAGTA69300.016.33525710
GCAGAGT76400.015.4042069
GTGTACG3402.3464963E-1015.264041
GTGGTAT12650.014.7693171
Produced by FastQC (version 0.11.5)