FastQCFastQC Report
Mon 6 Feb 2017
SRR2079180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086259
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.040.2655641
GTATCAA69850.039.7529831
TATCAAC97300.028.4087732
ATCAACG99900.028.0148433
TCAACGC99700.027.8979784
CAACGCA103800.026.7960345
AACGCAG106650.025.6979276
ACGCAGA125000.021.423217
CGCAGAG127300.020.7623678
AGAGTAC119900.019.82757211
GAGTACT86300.019.55093612
CAGAGTA126550.019.03105210
AGTACTT90800.018.61547713
GCAGAGT132900.018.4073269
GTACATG76950.018.334631
GTACTTT91300.018.29187214
TACATGG75300.018.1023482
GTGGTAT11700.017.7331541
ACATGGG81300.016.4692573
CGTGCGG1958.982277E-615.92492510
Produced by FastQC (version 0.11.5)