FastQCFastQC Report
Mon 6 Feb 2017
SRR2079179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122630
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.032.431971
GTATCAA40600.032.3841251
TCAACGC48100.026.4668084
ATCAACG49550.026.2493153
CAACGCA50650.024.9980955
TATCAAC53250.024.578232
AACGCAG52450.024.0086466
GTACATG79000.020.9351181
TACATGG77650.020.4127862
ACGCAGA61750.019.6096387
ACATGGG81900.018.9139733
CGCAGAG64450.018.8419638
AGAGTAC63500.017.06399211
CATGGGG47200.016.1536464
GCAGAGT73550.015.5306959
CAGAGTA71400.015.514276510
TAGCCCT19700.015.0609564
GTGTAGC16600.014.7987451
TACACTG22850.014.3435855
TATACTG12550.014.2948615
Produced by FastQC (version 0.11.5)