FastQCFastQC Report
Mon 6 Feb 2017
SRR2079179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122630
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61700.039.29061
GGTATCA26550.036.9921571
TATCAAC86650.027.562322
ATCAACG88100.027.2653853
TCAACGC88250.027.1408254
CAACGCA90500.026.08475
AACGCAG94000.024.9342756
ACGCAGA111300.020.7424747
CGCAGAG112300.020.431258
GAGTACT76350.019.43146312
CGCTATA1108.2207663E-418.825082
AGAGTAC111150.018.6556811
TACATGG75250.018.2082
GTACATG78200.018.1314661
AGTACTT82750.018.00882313
CAGAGTA118750.017.72320710
GTACTTT83800.017.49866714
CGTCGTA1200.001476930717.25107210
GCAGAGT123950.017.2023629
ACATGGG78450.016.8053933
Produced by FastQC (version 0.11.5)