FastQCFastQC Report
Mon 6 Feb 2017
SRR2079178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027408
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.032.0866661
GTATCAA45250.032.042781
ATCAACG56850.025.4856113
TCAACGC56550.025.3158024
CAACGCA58150.024.9158575
TATCAAC56600.024.7080522
AACGCAG60650.023.718196
ACGCAGA69550.019.5422847
GTACATG87150.019.1784971
CGCAGAG71400.018.7946768
TACATGG88200.018.674532
AGAGTAC68300.017.23073211
ACATGGG91850.016.9760063
GTGTAGC14900.016.257231
CAGAGTA74150.016.15058910
GCAGAGT77950.015.9828419
GTATAGC7200.015.86047651
GTGGTAT12500.015.5028951
TAGCCCT17750.015.3534154
GTACTTT32950.014.97615814
Produced by FastQC (version 0.11.5)