FastQCFastQC Report
Mon 6 Feb 2017
SRR2079178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027408
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.039.273661
GTATCAA63400.038.347131
TATCAAC88600.027.5027242
TCAACGC91450.026.9098034
ATCAACG91750.026.8970533
CAACGCA95050.025.89065
AACGCAG98700.024.5907696
TCGATCG1004.341474E-420.69687841
ACGCAGA115900.020.546687
CGCAGAG115750.020.2724638
GAGTACT75400.019.49618712
GTACTTT77050.019.03201114
AGAGTAC113450.018.82771311
GTACATG84350.018.8189071
TCGCGTC2608.913048E-1118.57872417
TACATGG85150.018.483532
AGTACTT79500.018.39844113
CAGAGTA120200.018.25845510
GCAGAGT123000.017.8989269
ACATGGG89300.017.315353
Produced by FastQC (version 0.11.5)