FastQCFastQC Report
Mon 6 Feb 2017
SRR2079177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834929
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35300.033.581671
GGTATCA18250.031.8148041
TCAACGC43200.026.5146224
ATCAACG43650.026.0041543
CAACGCA43650.025.8460735
TATCAAC44850.025.1687562
AACGCAG46550.024.23596
ACGCAGA54800.020.2713837
CGCAGAG56000.019.4673638
GTACATG67550.018.9815391
TACATGG67800.017.6675452
AGAGTAC54750.017.65055311
CAGAGTA59250.016.60125410
GCAGAGT63300.016.3021249
ACATGGG70900.016.2529163
CGATCGT1300.002524846515.92126842
GAGTACT27150.015.63412912
GAGTACA50950.015.465841
CTATACT9700.014.9385574
GTACTTT29350.014.93149714
Produced by FastQC (version 0.11.5)