FastQCFastQC Report
Mon 6 Feb 2017
SRR2079177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834929
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52450.039.896151
GGTATCA21100.037.702181
TCAACGC72300.028.594424
ATCAACG73000.028.5093463
TATCAAC72950.028.3396362
CAACGCA73650.027.882845
AACGCAG76350.026.856346
ACGCAGA90450.022.4344587
CGCAGAG91450.021.8444988
AGAGTAC88600.020.44432411
GAGTACT63600.020.18055212
GTACATG63900.019.5942571
CAGAGTA94650.019.46560110
AGTACTT67600.019.08514613
GCAGAGT98550.019.0803819
TACATGG65350.018.6960752
GTACTTT69600.018.24255814
ACATGGG68650.017.9481813
ACTTTTT85400.016.28149816
TACTTTT82500.015.47372915
Produced by FastQC (version 0.11.5)